| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stauffer, Peyton K (12)
|
MAUN-3 |
41.39 |
40.17 |
-1.22 |
| 2 |
Thoennes, Emma R (11)
|
MAPENN |
|
40.40 |
- |
| 3 |
Yushan, Rachel (12)
|
MAPVA |
40.07 |
40.74 |
0.67 |
| 4 |
Rasmussen, Charlotte B (11)
|
MAFWAC |
|
41.80 |
- |
| 5 |
Tran, Anna (12)
|
MAPVA |
42.24 |
42.46 |
0.22 |
| 6 |
Petrov, Sofia G (12)
|
MAPVA |
45.38 |
42.68 |
-2.70 |
| 7 |
Dieckmann, Mattea A (12)
|
MASJAC |
|
44.13 |
- |
| 8 |
Sutherland, Amelia V (12)
|
MAPENN |
|
44.45 |
- |
| 9 |
Kennedy, Emily L (11)
|
MAPENN |
|
45.08 |
- |
| 10 |
Do, Kaitlynn (12)
|
MAPVA |
45.19 |
45.46 |
0.27 |
| 11 |
Sharkey, Shannon M (12)
|
MAECCC |
52.38 |
45.78 |
-6.60 |
| 12 |
Zheng, Cindy C (12)
|
MASJAC |
|
46.17 |
- |
| 13 |
Baron, Kalyn F (11)
|
MAECCC |
47.86 |
46.50 |
-1.36 |
| 14 |
Chen, Jasmine (12)
|
MAPVA |
47.22 |
48.14 |
0.92 |
| 15 |
Torres-Thorpe, Charlie A (11)
|
MASJAC |
53.25 |
48.83 |
-4.42 |
| 16 |
Davis, Kennedy N (11)
|
MASJAC |
|
49.11 |
- |
| 17 |
Montuori, Ava R (11)
|
MAWAVE |
54.09 |
49.88 |
-4.21 |
| 18 |
DeNucci, Francesca T (11)
|
MASJAC |
54.28 |
50.47 |
-3.81 |
| 19 |
Russell, Miranda K (12)
|
MAYBCC |
59.21 |
53.80 |
-5.41 |
| 20 |
Leonard, Amber L (11)
|
MAPENN |
|
1:00.27 |
- |
| 21 |
Covill, Jillian S (11)
|
MAPENN |
|
1:03.92 |
- |
|
Yankovitch, Natalie L (11)
|
MAPVA |
48.61 |
DQ |
- |